"Drinking in the Dark" (DID): a simple mouse model of binge-like alcohol intake
- PMID: 24984686
- PMCID: PMC4142649
- DOI: 10.1002/0471142301.ns0949s68
"Drinking in the Dark" (DID): a simple mouse model of binge-like alcohol intake
Abstract
One of the greatest challenges that scientists face when studying the neurobiology and/or genetics of alcohol (ethanol) consumption is that most preclinical animal models do not voluntarily consume enough ethanol to achieve pharmacologically meaningful blood ethanol concentrations (BECs). Recent rodent models have been developed that promote binge-like levels of ethanol consumption associated with high BECs (i.e., ≥100 mg/dl). This unit describes procedures for an animal model of binge-like ethanol drinking which has come to be called "drinking in the dark" (DID). The "basic" variation of DID involves replacing the water bottle with a bottle containing 20% ethanol for 2 to 4 hr, beginning 3 hr into the dark cycle, on cages of singly-housed C57BL/6J mice. Using this procedure, mice typically consume enough ethanol to achieve BECs >100 mg/dl and to exhibit behavioral evidence of intoxication. An alternative two-bottle (ethanol and water) procedure is also described.
Keywords: binge-like; consumption; drinking-in-the-dark; ethanol; limited-access.
Copyright © 2014 John Wiley & Sons, Inc.
Figures
References
LITERATURE CITED
-
- Crabbe JC, Cameron AJ, Munn E, Bunning M, Wahlsten D. Overview of mouse assays of ethanol intoxication. Curr Protoc Neurosci. 2008;Chapter 9(Unit 9):26. - PubMed
KEY REFERENCE
-
- Rhodes JS, Best K, Belknap JK, Finn DA, Crabbe JC. Evaluation of a simple model of ethanol drinking to intoxication in C57BL/6J mice. Physiol Behav. 2005;84:53–63. - PubMed
Publication types
MeSH terms
Substances
Grants and funding
- R01 AA016789/AA/NIAAA NIH HHS/United States
- AA13519/AA/NIAAA NIH HHS/United States
- P50 AA010760/AA/NIAAA NIH HHS/United States
- R01 AA022048/AA/NIAAA NIH HHS/United States
- R01 AA013573/AA/NIAAA NIH HHS/United States
- R37 AA013573/AA/NIAAA NIH HHS/United States
- P60 AA007611/AA/NIAAA NIH HHS/United States
- P60 AA010760/AA/NIAAA NIH HHS/United States
- I01 BX000313/BX/BLRD VA/United States
- AA020245/AA/NIAAA NIH HHS/United States
- R01 AA015148/AA/NIAAA NIH HHS/United States
- AA015148/AA/NIAAA NIH HHS/United States
- U01 AA013519/AA/NIAAA NIH HHS/United States
- AA022048/AA/NIAAA NIH HHS/United States
- R24 AA020245/AA/NIAAA NIH HHS/United States
- AA016789/AA/NIAAA NIH HHS/United States
- AA013573/AA/NIAAA NIH HHS/United States
- AA10760/AA/NIAAA NIH HHS/United States
LinkOut - more resources
Full Text Sources
Other Literature Sources
