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. 2014 Jul 1:68:9.49.1-9.49.12.
doi: 10.1002/0471142301.ns0949s68.

"Drinking in the Dark" (DID): a simple mouse model of binge-like alcohol intake

Affiliations

"Drinking in the Dark" (DID): a simple mouse model of binge-like alcohol intake

Todd E Thiele et al. Curr Protoc Neurosci. .

Abstract

One of the greatest challenges that scientists face when studying the neurobiology and/or genetics of alcohol (ethanol) consumption is that most preclinical animal models do not voluntarily consume enough ethanol to achieve pharmacologically meaningful blood ethanol concentrations (BECs). Recent rodent models have been developed that promote binge-like levels of ethanol consumption associated with high BECs (i.e., ≥100 mg/dl). This unit describes procedures for an animal model of binge-like ethanol drinking which has come to be called "drinking in the dark" (DID). The "basic" variation of DID involves replacing the water bottle with a bottle containing 20% ethanol for 2 to 4 hr, beginning 3 hr into the dark cycle, on cages of singly-housed C57BL/6J mice. Using this procedure, mice typically consume enough ethanol to achieve BECs >100 mg/dl and to exhibit behavioral evidence of intoxication. An alternative two-bottle (ethanol and water) procedure is also described.

Keywords: binge-like; consumption; drinking-in-the-dark; ethanol; limited-access.

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Figures

Figure 1
Figure 1
A side (top) and aerial (bottom) view of the mouse cage set up for the basic one-bottle DID procedure. Note the Acco clip used to secure the ball-bearing sipper tube to the wire cage lid. Calibrations on the sipper tubes are aimed upwards so that the experimenter can record measures over the course of ethanol access.
Figure 2
Figure 2
Components needed to construct a ball-bearing sipper tube (left side) and constructed sipper tube (right side). Components shown, from top to bottom (left side) are a 10 ml disposable clear polystyrene serological pipet, a stainless steel ball-bearing sipper tube, heat shrink tubing, and a silicone stopper. Please see Supporting Procedures for details on the construction process.

References

LITERATURE CITED

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KEY REFERENCE

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