A functional Python bioinformatics tool for analyzing and ranking CRISPR guide RNAs (gRNAs) based on off-target specificity using NCBI BLAST
This project was created to help students evaluate the specificity of CRISPR-Cas9 gRNAs by automating off-target analysis through BLAST, then scoring and ranking the results with customizable logic.
- Clone this repo:
git clone https://github.com/MisplacedOrange/gRNAlyticscd crispr-grna-analyzer
- Install dependencies:
pip install biopython numpy- Make sure AchillesCommonEssentialControls.csv is in the same folder.
- Run the code!